| Group | Home | Away | ||
| E | Debreceni | 0-1 | Liverpool FC | watch video |
| E | ACF Fiorentina | 1-0 | Olympique Lyonnais | watch video |
| F | FC Rubin Kazan | 0-0 | FC Dynamo Kyiv | watch video |
| F | FC Barcelona | 2-0 | FC Internazionale Milano | watch video |
| G | Rangers FC | 0-2 | VfB Stuttgart | watch video |
| G | FC Unirea Urziceni | 1-0 | Sevilla FC | watch video |
| H | AZ Alkmaar | 0-0 | Olympiacos FC | watch video |
| H | Arsenal FC | 2-0 | R. Standard de Liège | watch video |
Matchday 5 - Wednesday 25 November 2009
| Group | Home | Away | ||
| A | FC Girondins de Bordeaux | 2-0 | Juventus | watch video |
| A | FC Bayern Munchen | 1-0 | Maccabi Haifa FC | watch video |
| B | PFC CSKA Moskva | 2-1 | VfL Wolfsburg | watch video |
| B | Manchester United FC | 0-1 | Beşiktaş JK | watch video |
| C | Real Madrid CF | 1-0 | FC Zürich | watch video |
| C | AC Milan | 1-1 | Olympique de Marseille | watch video |
| D | FC Porto | 0-1 | Chelsea FC | watch video |
| D | APOEL FC | 1-1 | Club Atlético de Madrid | watch video |
Group stage (red=out):
| Group A | Pld | Pts | Group B | Pld | Pts | |
| Bordeaux (FR) | 5 | 13 | Manchester United (UK) | 5 | 10 | |
| Juventus (IT) | 5 | 8 | Wolfsburg (DE) | 5 | 7 | |
| Bayern (DE) | 5 | 7 | CSKA Moskva | 5 | 7 | |
| Maccabi Haifa (IL) | 5 | 0 | Besiktas (TR) | 5 | 1 | |
| Group C | Pld | Pts | Group D | Pld | Pts | |
| Milan (IT) | 5 | 7 | Chelsea (UK) | 5 | 13 | |
| Real Madrid (ES) | 5 | 7 | Porto (PT) | 5 | 9 | |
| Marseille (FR) | 5 | 6 | Atletico (ES) | 5 | 3 | |
| Zurich (CH) | 5 | 3 | APOEL (CY) | 5 | ||
| Group E | Pld | Pts | Group F | Pld | Pts | |
| Fiorentina (IT) | 5 | 12 | Barcelona (ES) | 5 | 8 | |
| Lyon (FR) | 5 | 10 | Internazionale (IT) | 5 | 6 | |
| Liverpool (UK) | 5 | 7 | Rubin (RU) | 5 | 6 | |
| Debrecen (HU) | 5 | 0 | Dynamo (UA) | 5 | 5 | |
| Group G | Pld | Pts | Group H | Pld | Pts | |
| Sevilla (ES) | 5 | 10 | Arsenal (UK) | 5 | 13 | |
| Unirea Urziceni (RO) | 5 | 8 | Olympiacos (GR) | 5 | 7 | |
| Stuttgart (DE) | 5 | 6 | Standard (BE) | 5 | 4 | |
| Rangers (UK) | 5 | 2 | AZ (NL) | 5 | 3 | |
